| Solution Information | help | |
| Enzyme: | Nucleotide-binding oligomerization domain-containing protein 1 | |
| inhibitor: | BDBM62170 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay materials: 1) HEK-293-T NFKB-Luc cell line obtained from the assay provider's laboratory. 2) g-tri-DAP (Ana Spec cat #60774) obtained from assay provider's laboratory. 3) SteadyGlo (Promega) Protocol: 1. Compounds were diluted in DMEM 10% FBS plus Pen/Strep (culture medium), and 10 uL were added into respective wells (96-well culture plate) to reach desired final concentrations. 2. G-tri-DAP was added, in a final concentration of 5,4 ug/ml, to a culture medium suspension of HEK-293-T NFKB-Luc cells (1,1x10+6 cells per mililiter). 3. Pre-induced HEK-293-T NFKB-Luc cells were added into the respective wells (90 ul per well) and kept at 37oC (10% CO2 incubator) for 18 hours. 4. After incubation, plated cells were equilibrated to room temperature for 15 minutes. 5.Luciferase assay was then performed by adding 40 microliters per well of the Steady-GloTM reagent. After a ten minute incubation at room temperature, light emission was measured with the AnalystTM Microplate re | |
| If you find an error in this entry please send us an E-mail | ||